vol vol triton x 100 chloroform solution  (Valiant Co Ltd)

Journal: Parasites & Vectors

Article Title: Extracellular traps, an ancient defense mechanism described in hemocytes of the tick Rhipicephalus microplus

doi: 10.1186/s13071-025-07165-4

Figure Lengend Snippet: Kinetics of hemocyte chromatin release treated with different ETosis inducers. Chemical inducers: A A23817 and B PMA. Biological inducers: C LPS; D PGN; and E zymosan A. F Bacterial inducer: E. coli cells. The kinetics was performed from 0 to 180 min. Untreated hemocytes were used as a negative control, and 10% Triton X-100 was used as a positive control to release DNA through cellular lysis. The magenta line indicates the optimal concentration of the inducer. RFU, relative fluorescence units. Two-way ANOVA with Tukey’s multiple comparisons test post hoc was performed. The significant difference ( **** P < 0.0001) refers to the comparison between hemocytes treated with the best concentration inducing chromatin release and untreated hemocytes (control group)

Article Snippet: A 10% Triton X-100 solution (Thermo Scientific, Waltham, MA, USA) was used as a positive control for DNA released by cellular lysis, and untreated hemocytes (without inducer treatment) served as a negative control.

Techniques: Negative Control, Positive Control, Lysis, Concentration Assay, Fluorescence, Comparison, Control

Journal: Parasites & Vectors

Article Title: Extracellular traps, an ancient defense mechanism described in hemocytes of the tick Rhipicephalus microplus

doi: 10.1186/s13071-025-07165-4

Figure Lengend Snippet: Determination of pxn relative expression by sqRT–PCR in tick hemocytes at early times of chromatin release. All treatments showed significant differences compared with untreated hemocytes, except 10% Triton X-100. The R. microplus pghpx was used as an endogenous control for normalizing the pxn gene’s relative expression. One-way ANOVA with Tukey’s multiple comparison post hoc test compared with control. ** P < 0.001; ns, not significant

Article Snippet: A 10% Triton X-100 solution (Thermo Scientific, Waltham, MA, USA) was used as a positive control for DNA released by cellular lysis, and untreated hemocytes (without inducer treatment) served as a negative control.

Techniques: Expressing, Control, Comparison

Journal: Parasites & Vectors

Article Title: Extracellular traps, an ancient defense mechanism described in hemocytes of the tick Rhipicephalus microplus

doi: 10.1186/s13071-025-07165-4

Figure Lengend Snippet: Representative LCSM and TEM micrographies of tick hemocytes treated with zymosan A at early times before chromatin release (15 min). LSCM hemocytes stained with DAPI: A untreated; B zymosan A (0.01 µM); and C 10% Triton X-100 (scale bar, 20 μm). TEM: D untreated; E zymosan A (0.01 µM); and F 10% Triton X-100; (n, nuclei; v, vacuoles; m, mitochondria; g, electron-dense granules; * heterochromatin; ** euchromatin)

Article Snippet: A 10% Triton X-100 solution (Thermo Scientific, Waltham, MA, USA) was used as a positive control for DNA released by cellular lysis, and untreated hemocytes (without inducer treatment) served as a negative control.

Techniques: Staining